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Applications of BBa_K743014
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[http://2012.igem.org/Team:UC_Chile Team UC_Chile]
We tested this device in Synechocystis PCC6803 depending on the addition of two aldehydes substrates: decanal and dodecanal.
Expression was measured on a Termo Scientific Luminoskan Ascent luminometer for microplates. For more details on the experimental design go to Promoters Luciferase Assay at our [http://2012.igem.org/Team:UC_Chile/Protocols protocols page].
Upon addition, decanal is used as a substrate for the LuxAB luciferase reaction in E.coli cells transformed with the LuxBrick. Synechocystis cells transformed with K743015 don´t show luminescence, indicating that probably the Pta promoter is not being recognized by the transcriptional machinery.
Upon addition, dodecanal is used as a substrate for the LuxAB luciferase reaction in E.coli cells transformed with the LuxBrick. Synechocystis cells transformed with K743015 don´t show luminescence, indicating that probably the Pta promoter is not being recognized by the transcriptional machinery.
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UNIQff685af4964bdfcd-partinfo-00000000-QINU
UNIQff685af4964bdfcd-partinfo-00000001-QINU
UNIQff685af4964bdfcd-partinfo-00000002-QINU
